Coding

Part:BBa_K2736100:Design

Designed by: Lorenzo Pattiradjawane   Group: iGEM18_Utrecht   (2018-10-09)


eYFP::CheY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains a flexible glycine linker between the eYFP and CheY part


Source

This part was synthesized with Gibson assembly. The CheY fragment was ordered on IDT. The eYFP (BBa_I15017) fragment was isolated from the iGEM DNA distribution Kit. PCR with extended primers was used to create an overlap for Gibson assembly to stitch the fragments together.

References